产肠毒素大肠杆菌K88黏附素蛋白亚基Fae G在L.lactis中的表达及其抗原性

在构建L.lactis表达载体pNZ8148-fae G的基础上,将该载体转化到L.lactis NZ9000中,重组菌经5 μg/Lnisin诱导3 h,SDS-PAGE结果显示,FaeG在L.lactis中表达的目的蛋白相对分子质量大小约为27 000,表达产物的量达菌体可溶性总蛋白的10.89%.Western blot分析结果表明,该目的蛋白具有良好反应原性.将重组L.lactis口服免疫BALB/c小鼠,小鼠产生特异性IgG及黏膜slgA.本试验为进一步研究仔猪口服重组L.lactis疫苗,诱导其产生抗ETEC K88黏膜免疫的同时,发挥L.lactis的益生功能,预防仔猪腹泻奠定了基础.     

恩诺沙星口服混悬液的研制及其对仔猪黄白痢疗的效观察

以恩诺沙星为原料,纯化水为溶媒,利用胶体磨采用分散法研制出恩诺沙星口服混悬液,并对制剂进行了稳定性研究.结果显示,所有参试样品的外观性状、沉降体积比、再分散性、相对含量等指标均无明显变化,表明该制剂稳定性良好.结果表明,试验组与对照组对仔猪黄白痢的治愈率分别为72.0%和66.0%,有效率分别为92.0%和72.0%,试验组对仔猪黄白痢的有效率与对照组比较差异极显著(P＜0.01),表明恩诺沙星口服混悬液对仔猪黄白痢的临床疗效显著优于恩诺沙星溶液.     

加强国营林场生产建设,充分发挥骨干示范作用

党的十一届三中全会以后,国营林场的生产建设发展更快,仅1988年全省国营林场完成造林80O0公顷,生产木材34.68万m~3,上交国家税收2461万元,经营纯收入4197万元,经费自给率达62.5％。实践证明,兴办国营林场是国家投资少,收效大的有效途径。但是,当前国营林场在经营观念、管理体制,资源开发利用,管理水平等方面,仍存在不少问题,影响其发展能力、本文就国营林场如何转变指导思想,增加后续资源,加快森林资源开发利用,改革管理体制、筹集开发建设资金、加强科技工作等方面,提出作者的看法和意见,以供参考。     

口服补液、抗菌止泻药物治疗牛、马腹泻的试验研究

对４０例牛、马原发性肠炎和消化不良性腹泻临床病例进行了口服补液、抗菌止泻药物（口服补液盐液、痢菌净及泻速宁２号）治疗试验。结果表明：口服补液盐液各成份均可吸收，具有补充水、葡萄糖、钠、钾、氯及碳酸氢盐的作用；口服补液盐液与抗菌止泻药物配伍，有协同作用，使用简便、安全。     

网络农业信息分类和编码的研究

信息分类的标识是信息管理的重要手段。作者采用行业与信息属性结合的方法，提出了一种农业信息分类的方案，并进行了信息编码的设计。     

全国森林公安机关数字文献资源应用及共享需求调查分析

采用调查问卷方式,对全国森林公安数字文献资源应用现状及共享需求进行调查,对回收的数据做统计分析和归纳总结认为:在森林公安机关单位,虽然互联网数字资源的应用日趋明显,但传统纸质文献的运用还相当普遍;对数字资源的使用还有一定局限,获取渠道比较单一;在数字文献资源的建设和应用上还存在资金、人才、设施等诸多困难.为此,需进一步加大宣传普及力度,统一规划,多方努力,共同挖掘潜力,建立和完善共享机制.     

试论农业科研单位内部控制制度的实施

分析了农业科研单位实施内部控制的现状与存在问题,明确了内部控制制度的重要性,指出只有有效实施内部控制才能使科学研究正常运转并处于良好状态,最后提出了在农业科研单位实施内部控制制度的几点建议。     

贵州省林业行政案件发生的主要原因分析及对策探讨

对贵州省“十五”林业行政案件发生的原因进行了认真分析,并就进一步加强和改进森林资源保护管理及林业行政执法工作进行了初步探讨。     

创新管理机制确保信息服务产业化的实现

文中阐述了传统信息服务业向现代信息服务产业转变过程中，两院信息中心(即中国热带农业科学院、华南热带农业大学信息中心)存在的内部机制问题及工作现状，进而，从创新管理机制的角度探讨深化改革的措施。     

A RETROSPECTIVE ANALYSIS OF 140 DOGS WITH ORAL MELANOMA TREATED WITH EXTERNAL BEAM RADIATION

Despite the early notion that canine oral malignant melanoma is radioresistant, recent data suggest that external beam radiotherapy is effective in local tumor control. However, optimal fractionation schedules have not been established. The high rate of regional and distant metastasis is another problem that has hindered long-term control. The role of chemotherapy in the management of canine oral melanoma has also not been determined. In this study, data from 140 dogs irradiated at North Carolina State University were evaluated with the following objectives: (1) to compare the efficacy of three radiation therapy protocols (36 Gy, 9 Gy x 4 fractions; 30 Gy, 10 Gy x 3 fractions; or >45 Gy, 2-4 Gy x 12-19 fractions) for the treatment of dogs with oral malignant melanoma, (2) to identify any host or tumor factors influencing prognosis, and (3) to determine the impact of systemic chemotherapy on treatment outcome. Information regarding response to therapy, disease progression, and survival were determined from the medical records or from information obtained by telephone or mail survey. Relationships between host, tumor, and treatment variables and outcome measures (response, time to first event, and survival) were evaluated using Fisher's exact test (response) and the Cox regression model (time to first event and survival). The median time to first event for the 140 dogs was 5.0 months (95% C.I., 4-6 months) and the median survival was 7.0 months (95% C.I., 6-9 months). In the univariate analysis, the following variables were associated with increased time to first event and survival: (1) rostral tumor sublocation; (2) lack of bone lysis observed on skull imaging, and (3) microscopic tumor burden. In a multivariate analysis of 111 dogs with complete data for these variables, tumor sublocation, bone lysis, and tumor volume were identified as joint predictors of time to first event (p < .001, p < .001, and p = .04, respectively) and survival (p < .001, p < .001, and p = .05, respectively). There were no differences in response, time to first event and survival between the three radiation therapy protocols used. Systemic chemotherapy had no impact on the development of metastatic disease, time to first event, or survival, although the dosages used in this study were suboptimal. External beam radiation therapy is effective in local disease control of canine oral malignant melanoma; however, the optimal fractionation scheme has yet to be determined. The high metastatic rate observed with this disease and the inefficacy of systemic chemotherapy indicate that further investigation into novel therapies is warranted.